The best time to take ashwagandha can be anytime during the day. Ashwagandha can be taken with or without food, but you may want to split your doses up to take in the morning and night. However, studies have found that taking ashwagandha before bed may improve sleep quality by helping you fall asleep and achieve REM sleep faster [1].
References
Langade, D., Kanchi, S., Salve, J., Debnath, K., & Ambegaokar, D. (2019). Efficacy and safety of ashwagandha (Withania somnifera) root extract in insomnia and anxiety: A double-blind, randomized, placebo-controlled study. Cureus. https://doi.org/10.7759/cureus.5797
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